Journal: Neurobiology of Pain

Article Title: Human-like cutaneous neuropathologies associated with a porcine model of peripheral neuritis: A translational platform for neuropathic pain

doi: 10.1016/j.ynpai.2018.07.002

Figure Lengend Snippet: Representative images of Cy3 immunofluorescent labeling (red) for Nav1.7 (left column), ETA (middle column), and ETB (right column) in two Sham28 (A–F) and two PNT28 (G–L) ipsilateral biopsies. Cell nuclei are counterstained with DAPI (blue fluorescence). E, epidermis; UD, upper dermis; SB, Stratum Basalis; SS, Stratum Spinosum; SG, Stratum Granulosum; SC, Stratum Corneum. Scale bar = 100 μm. A,D,G,J. Nav1.7 immunolableing in Sham28 biopsies was concentrated in SG and upper SS (brackets in A, D) and increased in intensity as well as expanding through the full depth of SS, encroaching on SB in PNT28 biopsies (brackets in G, J). Quantification was based on repeated sampling of average PI in the upper and lower keratinocytes demarcated by the broken and dotted line marquees, respectively. (B, E, H, K) ETA expression in Sham28 biopsies was concentrated in SB (brackets in B, E) and expanded through the full depth of SG and SS in PNT28 biopsies (brackets in H, K). Quantification was based on repeated sampling of average pixel intensities in the SG and SS and in SB demarcated by the broken and dotted line marquees, respectively. (C, F, I, L) ETB expression in Sham28 biopsies was concentrated in SG and upper SS (brackets in C, F) and diminished in PNT28 biopsies (brackets in I, L). Quantification was based on repeated sampling of average pixel intensities in the SG and SS demarcated by the broken line marquees. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Three additional slides from each biopsy were also prepared to detect keratinocyte immunolabeling with specific antibodies against: 2. voltage-gated sodium channel alpha subunit 1.7 ( Nav1.7 ; rabbit polyclonal, 1:500, Alomone).

Techniques: Labeling, Fluorescence, Sampling, Expressing

Journal: Neurobiology of Pain

Article Title: Human-like cutaneous neuropathologies associated with a porcine model of peripheral neuritis: A translational platform for neuropathic pain

doi: 10.1016/j.ynpai.2018.07.002

Figure Lengend Snippet: Keratinocyte average immunofluorescent pixel intensity (PI) from FC18, PC10, PC28, PNT28, and Sham28 for Nav1.7 (A), ETA (C), CGRP (E), and ETB (F). * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.005. (B) Ratio of lower to upper average PI for Nav1.7 (black and gray bars respectively). (D) Ratio of combined SG and SS to SB average PI for ETA (black and gray bars respectively).

Article Snippet: Three additional slides from each biopsy were also prepared to detect keratinocyte immunolabeling with specific antibodies against: 2. voltage-gated sodium channel alpha subunit 1.7 ( Nav1.7 ; rabbit polyclonal, 1:500, Alomone).

Techniques:

Journal: Neurobiology of Pain

Article Title: Human-like cutaneous neuropathologies associated with a porcine model of peripheral neuritis: A translational platform for neuropathic pain

doi: 10.1016/j.ynpai.2018.07.002

Figure Lengend Snippet: (A) Average keratinocyte Nav1.7 PI among upper and lower keratinocytes for ipsilateral biopsies (solid black and gray bars, respectively) and contralateral biopsies (open black and gray bars, respectively) from PNT1, PNT7, PNT14, and PNT 21 pigs (see , Part 2). (B) Ratios of lower divided by upper average keratinocyte Nav1.7 PI for ipsilateral and contralateral biopsies (solid and open bars, respectively). (A) and (B) show results from average ipsilateral Nav1.7 PI among upper and lower epidermal keratinocytes in PNT28 biopsies (solid black and gray bars, respectively) and ipsilateral Sham28 biopsies (open broken line black and gray bars, respectively), included from A and B.

Article Snippet: Three additional slides from each biopsy were also prepared to detect keratinocyte immunolabeling with specific antibodies against: 2. voltage-gated sodium channel alpha subunit 1.7 ( Nav1.7 ; rabbit polyclonal, 1:500, Alomone).

Techniques:

Journal: Cell Reports Methods

Article Title: Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue

doi: 10.1016/j.crmeth.2024.100816

Figure Lengend Snippet:

Article Snippet: Ppp1r17 TaqMan Assay , Thermo Fisher Scientific , Cat#: Mm00495458_m1.

Techniques: Recombinant, Reverse Transcription, TaqMan Assay, Microscopy, Western Blot, Software